The nature and detection of mycoplasmal immunogens

Mycoplasmal infections are important causes of disease in cattle, swine, sheep, goats and poultry. Vaccination has been shown experimentally to induce protection against challenge with several mycoplasmas, and vaccines have been used to control naturally occurring mycoplasmal disease in swine, sheep, goats and poultry. Immune responses to mycoplasmal immunogens have been determined using ELISA and immunoblotting as well as other serologic techniques. However, the importance of specific immunogens as virulence factors or putative protective immunogens has generally not been determined. Investigations are underway to determine the pathogenic mechanisms and identify important virulence factors involved in mycoplasmal disease. Examples are discussed of investigations with Mycoplasma hyopneumoniae from our own laboratory. We have utilized ATP luminometry in attempts to develop better methods for quantitation of growth of M. hypopneumoniae and competitive ELISA as a potential method for in vitro quantitation of specific important immunogens.