APB alters photopic spectral sensitivity of the goldfish retina

Because the glutamate analog 2-amino-4-phosphonobutyric acid (APB) alters synaptic transmission at the outer plexiform layer in goldfish we asked whether intraocular injection of ABP would alter the spectral sensitivity of the retina. The spectral sensitivity of the ON and OFF components of the optic nerve response (ONR) in goldfish was measured in the presence and absence of APB, under four chromatic adaptation condition. APB decreased absolute sensitivity and altered spectral sensitivity for both ON and OFF responses under each adaptation condition. The spectral sensitivity of the OFF response was altered most at short wavelengths, in a manner consistent with a change in the balance of additive cone inputs. For the ON response, the effects of APB were consistent with a change in spectral antagonism, particularly between M- and L-cones. These results suggest that the activity in the retinal cone pathways in goldfish can be influenced by a mechanism incorporating an APB-sensitive receptor, and that this receptor may be intimately involved with setting the balance of cone inputs to spectrally-opponent neurons.