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Schwann cells transplanted into the CNS
G Raisman1, J M Lawrence, G A Brook
1Norman and Sadie Lee Research Centre, Laboratory of Neurobiology, National Institute for Medical Research, Mill Hill, London, U.K.
Summary
Transplanted Schwann cells migrated extensively within the rat brain, adopting unique forms and interacting with host cells. These cells did not form peripheral myelin, and their long-term survival remains uncertain.
Area of Science:
- Neuroscience
- Cell Biology
- Regenerative Medicine
Background:
- Schwann cells are glial cells of the peripheral nervous system.
- Their potential for migration and integration into the central nervous system is of therapeutic interest.
Purpose of the Study:
- To investigate the behavior and integration of purified rat Schwann cells transplanted into the adult rat hippocampus and fimbria.
Main Methods:
- Purified Schwann cells were cultured and injected into host brain regions.
- Cell identification used light/electron microscopy, antigenic profiling (low affinity nerve growth factor receptor), microsphere uptake, and [3H]thymidine autoradiography.
- Migration patterns and host responses (astrocytic hypertrophy) were analyzed.
Main Results:
- Transplanted Schwann cells exhibited distinct elongated morphology with long processes.
- Cells migrated significantly within the hippocampus and along the fimbria, interacting with host neural tissue.
- A transient host astrocytic hypertrophy was observed without hindering Schwann cell migration.
- Donor Schwann cells did not exhibit peripheral myelin (P0) immunoreactivity.
Conclusions:
- Purified Schwann cells can migrate extensively and integrate into the central nervous system environment after transplantation.
- The cells adopt unique morphologies and induce host astrogliosis.
- The capacity for myelination and long-term survival in the CNS requires further investigation.