Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Homogeneous sampling accounts for the increased diagnostic accuracy using the ThinPrep Processor

M L Hutchinson1, L M Isenstein, A Goodman

  • 1Department of Cytopathology, New England Medical Center, Tufts University, Boston, Massachusetts.

American Journal of Clinical Pathology
|February 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "A five-year multicentre evaluation of candidaemia in UK adults: Epidemiology, risk factors and outcomes" [J Infect 92 (2026) 106692].

The Journal of infection·2026
Same author

A five-year multicentre evaluation of candidaemia in UK adults: Epidemiology, risk factors and outcomes.

The Journal of infection·2026
Same author

Exploring environmental nanobiogeochemistry using field-flow fractionation and ICP-MS-based tools: background and fundamentals.

Environmental science. Nano·2025
Same author

Exploring environmental nanobiogeochemistry using field-flow fractionation and ICP-MS-based tools: progress and frontiers.

Environmental science. Nano·2025
Same author

Co-design of interventions to improve acute care in hospital: A rapid review of the literature and application of the BASE methodology, a novel system for the design of patient centered service prototypes.

Acute medicine·2023
Same author

Adult life-course trajectories of psychological distress and economic outcomes in midlife during the COVID-19 pandemic: evidence from the 1958 and 1970 British birth cohorts.

Social psychiatry and psychiatric epidemiology·2023
Same journal

Invasive urothelial carcinoma in association with surface low-grade papillary urothelial carcinoma: clinical and pathologic insights from a rare entity.

American journal of clinical pathology·2026
Same journal

CEACAM1 expression by immunohistochemistry in B-cell lymphomas and plasma cell myeloma.

American journal of clinical pathology·2026
Same journal

Comprehensive multicriteria life cycle assessment of biopsy processing in a surgical pathology department.

American journal of clinical pathology·2026
Same journal

Prognostic significance of Myb protein and its downstream target genes in lacrimal gland adenoid cystic carcinoma.

American journal of clinical pathology·2026
Same journal

Mismatch repair protein "nonclassic expression loss" pattern in colorectal cancer: an important staining pattern that is not well understood.

American journal of clinical pathology·2026
Same journal

Musculoskeletal pain in medical laboratory personnel: a cross-sectional study.

American journal of clinical pathology·2026
See all related articles

The ThinPrep method for Papanicolaou smear preparation offers superior diagnostic accuracy compared to conventional direct smears. This is because the ThinPrep method minimizes preparation errors by ensuring consistent cell transfer, unlike direct smears.

Area of Science:

  • Gynecologic pathology
  • Cervical cancer screening
  • Cytopathology

Background:

  • Clinical trials show ThinPrep method has higher diagnostic sensitivity than conventional direct Papanicolaou smears.
  • Reduced accuracy in conventional direct Pap smears may stem from nonhomogeneous cell sampling during slide transfer.

Purpose of the Study:

  • To investigate the hypothesis that preparation errors contribute to reduced accuracy of conventional direct Pap smears.
  • To compare cell deposition efficiency between four direct smear methods and the ThinPrep fluid-based, filter-transfer method.

Main Methods:

  • Four conventional direct smear techniques were compared against the ThinPrep fluid-based, filter-transfer method.
  • Epithelial cell counts were performed on conventional smears to quantify cell deposition.

Related Experiment Videos

  • Replicate slides were prepared per specimen using the ThinPrep method to assess consistency.

    • Main Results:

    • Conventional smears deposited only a fraction of available epithelial cells (medians, 6.5% to 62.5%) from sampling devices onto slides.
    • The ThinPrep method consistently yielded equivalent diagnostic material across replicate slides for all 27 cases studied.
    • Preparation error was identified as a significant source of inaccuracy in conventional Pap smears.

    Conclusions:

    • The ThinPrep method significantly reduces preparation error compared to conventional direct Pap smears.
    • Consistent cell transfer in the ThinPrep method ensures reliable diagnostic material for accurate cervical cancer screening.
    • The findings highlight the importance of sample preparation methods in improving the diagnostic accuracy of Papanicolaou smears.