Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intramembranous bone matrix is osteoinductive

C K Scott1, S D Bain, J A Hightower

  • 1Department of Cell Biology and Neuroscience, School of Medicine, University of South Carolina, Columbia 29208.

The Anatomical Record
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of muscle dysfunction on bone mass and morphology.

Journal of musculoskeletal & neuronal interactions·2010
Same author

Strontium ranelate improves bone strength in ovariectomized rat by positively influencing bone resistance determinants.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2008
Same author

Estrogen modulates estrogen receptor alpha and beta expression, osteogenic activity, and apoptosis in mesenchymal stem cells (MSCs) of osteoporotic mice.

Journal of cellular biochemistry. Supplement·2001
Same author

Increased marrow-derived osteoprogenitor cells and endosteal bone formation in mice lacking thrombospondin 2.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2000
Same author

The standard medical microscopic anatomy course: histology circa 1998.

The Anatomical record·1999
Same author

Mice that lack thrombospondin 2 display connective tissue abnormalities that are associated with disordered collagen fibrillogenesis, an increased vascular density, and a bleeding diathesis.

The Journal of cell biology·1998
Same journal

Gross and histological structure of the pharyngeal constrictors in the rabbit.

The Anatomical record·2014
Same journal

The amniotic duct as key to the direction of growth of the human placenta and its orientation in the uterus.

The Anatomical record·2010
Same journal

Mammary structure and secretion in premature and full-term infants.

The Anatomical record·2010
Same journal

Extrapyramidal projections from the cat's cerebral cortex.

The Anatomical record·2010
Same journal

The effect of colchicine on the rat embryo.

The Anatomical record·2010
Same journal

Lymphocytogenesis in human lymph nodes.

The Anatomical record·2010
See all related articles

Researchers isolated a novel osteoinductive factor from intramembranous (IM) bone, distinct from those found in endochondral (EC) bone. This discovery advances understanding of bone formation mechanisms and potential therapeutic targets.

Area of Science:

  • Biochemistry
  • Orthopedic Research
  • Bone Biology

Background:

  • Known osteoinductive factors are derived from endochondral (EC) bone, initiating bone formation via EC ossification.
  • No comparable factors have been isolated from intramembranous (IM) ossification-derived bones.
  • Understanding IM bone formation factors is crucial for regenerative medicine and skeletal repair.

Purpose of the Study:

  • To isolate and characterize osteoinductive proteins from IM bone.
  • To investigate potential differences between IM and EC bone-derived osteoinductive factors.

Main Methods:

  • Proteins were extracted from bovine frontal bone (IM origin) using guanidine hydrochloride.
  • A 10-100 kDa fraction was isolated via ultrafiltration and purified using heparin-Sepharose (HS) chromatography.

Related Experiment Videos

  • Purified factors were lyophilized with collagen, implanted in rats, and assessed for ectopic bone formation using radiology and microscopy.

    • Main Results:

    • An HS-binding osteoinductive factor was successfully extracted and partially purified from IM bone matrix.
    • The isolated IM bone factor exhibited a distinct SDS-PAGE banding pattern compared to EC bone factors.
    • Ectopic bone formation was confirmed at the implantation sites.

    Conclusions:

    • A novel osteoinductive factor can be isolated from IM bone matrix.
    • This IM bone-derived factor differs molecularly from known EC bone-derived factors.
    • Findings suggest distinct molecular mechanisms underlying IM and EC ossification.