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Use of solid-phase PCR for enhanced detection of airborne microorganisms
A J Alvarez1, M P Buttner, G A Toranzos
1Department of Biology, College of Natural Sciences, University of Puerto Rico, Rio Piedras 00931.
Applied and Environmental Microbiology
|January 1, 1994
Abstract:
The solid-phase PCR (SP-PCR) was compared with a culture-based technique for the detection of aerosolized Escherichia coli DH1. Results with SP-PCR showed an increase in detection sensitivity over that of culture methods. Therefore, SP-PCR may be useful for the detection of airborne microorganisms which may be nonculturable because of aerosolization or sampling stress.