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Isolation of a putative hydroxyacyl enzyme intermediate of an epoxide hydrolase
B D Hammock1, F Pinot, J K Beetham
1Department of Entomology, University of California, Davis.
Biochemical and Biophysical Research Communications
|February 15, 1994
Abstract:
A putative covalent, alpha-hydroxyacyl intermediate was isolated by the brief exposure of murine soluble epoxide hydrolase to its substrate. The reaction was reversed by time and blocked by competitive inhibitors. The formation of the intermediate was dependent upon the concentration of the enzyme and was increased by incubation under acidic conditions. The structure of the intermediate was supported by microchemical methods.