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Cloning and expression analysis of a viroid-induced peroxidase from tomato plants
P Vera1, P Tornero, V Conejero
1Departament of Biotechnology, Universidad Politécnia de Valencia, Spain.
Molecular Plant-Microbe Interactions : MPMI
|November 1, 1993
Abstract:
Differential hybridization was used to detect transcripts induced both by ethylene and by viroid infection in tomato plants. A cDNA clone encoding a putative peroxidase was isolated and characterized. DNA sequencing revealed high homology with a lignin-peroxidase from tobacco. Northern blot analysis showed specific induction of this peroxidase gene in viroid-infected plants. An increase in the level of mRNA accumulation is obtained by ethylene treatment, reinforcing the idea that ethylene is a mediator in the response of tomato plants to viroid infection.