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Synthesis and characterization of oligonucleotides containing the alpha-anomer of deoxyadenosine to study its
1Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Biochemistry and Molecular Biology International
|November 1, 1993
Abstract:
The alpha-anomer of 2'-deoxyadenosine 1 is a major adenine lesion produced by hydroxyl radicals in DNA. To assess its biochemical effects on DNA replication, 1 was site-specifically incorporated into oligonucleotide templates using phosphoramidite chemistry. The templates were purified and extensively characterized to ensure their homogeneity and nucleotide sequences.