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Related Experiment Videos

Rat microglial interleukin-3

P J Gebicke-Haerter1, K Appel, G D Taylor

  • 1Department of Psychiatry, University of Freiburg, Medical School, Germany.

Journal of Neuroimmunology
|March 1, 1994
PubMed
Summary
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Microglia, the immune cells of the brain, produce Interleukin-3 (IL-3), a growth factor that promotes their own proliferation. This finding suggests IL-3 plays a role in brain immune responses.

Area of Science:

  • Neuroimmunology
  • Cell Biology

Background:

  • Interleukin-3 (IL-3) is a hematopoietic growth factor.
  • Microglia, the central nervous system's resident macrophages, proliferate in response to IL-3.
  • Astrocytes were previously hypothesized to produce the microglial growth factor.

Purpose of the Study:

  • To investigate if microglial cells themselves are a source of IL-3.
  • To characterize IL-3 expression and its role in microglial proliferation.

Main Methods:

  • Primary rat microglial cell cultures.
  • Western blotting and immunoprecipitation to detect IL-3 protein.
  • In situ hybridization and RT-PCR to detect IL-3 mRNA.
  • Functional assays using anti-IL-3 antibodies.

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Main Results:

  • Microglial conditioned media enhanced microglial proliferation.
  • IL-3 protein and mRNA were detected in isolated microglia.
  • Anti-IL-3 antibodies inhibited microglial proliferation induced by conditioned media.
  • IL-3 mRNA expression was observed irrespective of lipopolysaccharide stimulation.

Conclusions:

  • Rat microglial cells are an endogenous source of IL-3.
  • IL-3 produced by microglia contributes to their proliferation.
  • Microglial IL-3 may be involved in recruiting immune cells to brain lesions.
  • Microglial IL-3 expression might compensate for T cell functions in the brain.