Jove
Visualize
Contact Us

Related Experiment Videos

T cell recognition of somatically-generated Ab diversity

M C Eyerman1, L Wysocki

  • 1Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.

Journal of Immunology (Baltimore, Md. : 1950)
|February 15, 1994
PubMed
Summary

T cell tolerance can recognize self-antibodies (Abs) with novel structures. T cell hybridomas specifically recognized somatically mutated antibody light chains, demonstrating T cell recognition of physiologically acquired mutations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

T cell recognition and tolerance of antibody diversity.

Journal of immunology (Baltimore, Md. : 1950)·1996
Same author

An ephemeral pheromone of female house mice: perception via the main and accessory olfactory systems.

Physiology & behavior·1995
Same author

NADPH diaphorase staining suggests localization of nitric oxide synthase within mature vertebrate olfactory neurons.

Neuroscience·1995
Same author

Lack of connectivity between the induced and autoimmune repertoires of lpr/lpr mice.

Immunology·1993
Same author

The amino acid residues at the VH-D-JH junctions affect the affinity of anti-p-azophenylarsonate antibodies.

Journal of immunology (Baltimore, Md. : 1950)·1989
Same author

Proinflammatory effects of interleukin 1 in the rat air pouch.

International journal of tissue reactions·1989
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Immunology
  • Molecular Biology
  • T cell biology

Background:

  • During immune responses, B cells generate antibodies (Abs) with novel structures via somatic hypermutation.
  • This process challenges T cell self-tolerance as mutated antibody V regions may not be represented in the T cell repertoire during thymic selection.

Purpose of the Study:

  • To investigate if T cells can recognize antibody V regions that have undergone somatic mutation.
  • To explore the potential for T cell recognition of self-antibodies generated during an immune response.

Main Methods:

  • Immunization of A/J mice with two somatically mutated monoclonal antibodies (mAbs).
  • Generation and characterization of 13 mAb-specific T cell hybridomas expressing alpha beta receptors and CD4.
  • Antigen presentation studies using fixed antigen-presenting cells (APCs) and whole or processed mAbs.

Related Experiment Videos

Main Results:

  • All T cell hybridomas responded in a class II MHC-restricted manner, requiring mAb processing.
  • All 13 T cell hybridomas recognized the somatically mutated light chains of the tested mAbs.
  • No T cell hybridoma responses were observed against the unmutated versions of the light chains.

Conclusions:

  • The T cell repertoire contains members capable of recognizing syngeneic antibodies with acquired somatic mutations.
  • This recognition occurs in a conventional class II MHC-restricted manner.
  • Somatic mutations in antibody genes can generate neo-epitopes recognized by T cells, impacting self-tolerance.