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Four polymorphic markers on rat chromosome 12 form a single linkage group
P Mathern1, E A Goldmuntz, H Zha
1Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Biochemical Genetics
|October 1, 1993
Summary
Researchers mapped four polymorphic markers to rat chromosome 12 using linkage analysis. These markers, identified via PCR, are highly polymorphic and useful for future rat genetic studies.
Area of Science:
- Genetics
- Molecular Biology
- Genomics
Background:
- Genetic mapping is crucial for understanding chromosome organization and function.
- Rat chromosome 12 harbors genes relevant to various physiological processes.
- Polymorphic markers are essential tools for genetic linkage analysis.
Purpose of the Study:
- To map four novel PCR-typable polymorphic markers to specific locations on rat chromosome 12.
- To establish the order and genetic distances between these markers.
- To assess the utility of these markers for broader genetic studies in rats.
Main Methods:
- Linkage analysis was performed on F2 intercross progeny from Fischer (F344/N) and Lewis (LEW/N) rat strains.
- Four simple sequence repeat markers were identified and genotyped using PCR.
- The plasminogen activator inhibitor (Planh) gene's known location on chromosome 12 facilitated the assignment of the linkage group.
Main Results:
- A single linkage group of 27.7 cM was established on rat chromosome 12.
- The order of markers was determined as Planh--Pepckr2--D12N155--Sdh.
- All four markers demonstrated high polymorphism across 13 diverse inbred rat strains.
Conclusions:
- The developed set of polymorphic markers provides valuable tools for genetic mapping in rats.
- These markers can aid in the identification of genes associated with complex traits and diseases in rat models.
- The established linkage map contributes to the overall understanding of rat chromosome 12 organization.