[Studies on cross reactions of antisera against nucleotid-protein conjugates with denaturated desoxyribonucleic...

The reactivity of anti-nucleotide-sera (anti-UMP, anti-AMP, anti-GMP) with denatured DNA's of 10 different sources was investigated by means of quantitative complement fixation. The haptene specificity of the anti-nucleotide antibodies was shown by haptene inhibition of the reaction with DNA. Anti-UMP showed cross-reactions with other pyrimidine-derivatives, but to a lesser extent; anti-GMP cross-reacted with adenine structures. Quantitative differences were observed in the reaction of anti-UMP, anti-AMP and anti-GMP with DNA's of various (G + C)-contents. These differences could not be correlated in all cases with the overall base composition of the DNA.