Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

GM2 ganglioside activator occurs in multiple forms

A Novak1, J A Lowden

  • 1Division of Neurosciences, Hospital for Sick Children, Toronto, Canada.

Biochimica Et Biophysica Acta
|March 2, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Light Pseudoscalar Bosons, Pair-Produced in Higgs Boson Decays in the Four-Electron Final State in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

First Evidence for Mixing-Induced CP Violation in B_{s}^{0}→J/ψϕ(1020) Decays in pp Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

Observation of Suppressed Charged-Particle Production in Ultrarelativistic Oxygen-Oxygen Collisions.

Physical review letters·2026
Same author

Measurement of D^{0} Meson Photoproduction in Ultraperipheral Heavy Ion Collisions.

Physical review letters·2026
Same author

Observation of tWZ Production at the CMS Experiment.

Physical review letters·2026
Same author

First Exclusive Reconstruction of the B^{*+}, B^{*0}, and B_{s}^{*0} Mesons and Precise Measurement of Their Masses.

Physical review letters·2026
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

Researchers purified the GM2 activator protein from human kidney, essential for hexosaminidase A activity. This protein exists in multiple glycosylated forms, as shown by SDS-PAGE analysis.

Area of Science:

  • Biochemistry
  • Glycobiology
  • Enzymology

Background:

  • Hexosaminidase A is crucial for GM2 ganglioside hydrolysis.
  • GM2 activator protein facilitates this enzymatic reaction.
  • Understanding the activator's properties is key to metabolic disease research.

Purpose of the Study:

  • To purify and characterize the GM2 activator protein from human kidney.
  • To investigate the molecular properties and potential heterogeneity of the GM2 activator.

Main Methods:

  • Protein purification from human kidney tissue.
  • Gel filtration chromatography to determine molecular mass.
  • SDS-PAGE to analyze subunits and heterogeneity.
  • Concanavalin A-Sepharose chromatography to assess glycosylation.

Related Experiment Videos

Main Results:

  • The GM2 activator was purified from human kidney.
  • Gel filtration indicated a molecular mass of 28 kDa.
  • SDS-PAGE revealed three major bands at 23, 22, and 21 kDa.
  • Differential binding to Concanavalin A-Sepharose indicated multiple glycosylated forms.

Conclusions:

  • The human kidney GM2 activator exists as multiple glycosylated forms.
  • This heterogeneity may influence its function in GM2 ganglioside hydrolysis.
  • Further studies are needed to elucidate the functional significance of these glycosylated variants.