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Analysis of FCE 23762 (methoxymorpholinodoxorubicin hydrochloride), a new antitumour agent, by HPTLC and scanning
A Farina1, M G Quaglia, A Doldo
1Istituto Superiore di Sanità, Roma, Italy.
Journal of Pharmaceutical and Biomedical Analysis
|November 1, 1993
Abstract:
A simple, rapid and reproducible high-performance thin-layer chromatographic (HPTLC) method using UV or fluorescence scanning densitometry has been developed for the assay and purity control of methoxymorpholinodoxorubicin hydrochloride (FCE 23762). With a mobile phase of chloroform-methanol-acetic acid (93:6:1, v/v/v) and a silica gel plate, all potential impurities were separated from the main component and from each other. Detection limits at a signal-to-noise ratio of 2:1 were a few nanograms for UV detection and < 1 ng for fluorescence emission. The RSD values for the examined compounds were all < 3%.