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Quantitative capillary electrophoresis and its application to the polyanionic quinobene

K Hettiarachchi1, A P Cheung

  • 1Life Sciences Division, SRI International, Menlo Park, CA 94025.

Journal of Pharmaceutical and Biomedical Analysis
|November 1, 1993
PubMed
Summary

This study enhances capillary electrophoresis (CE) accuracy by addressing reproducibility and reliability factors. Developed CE assays for quinobene and suramin demonstrate significant improvements in precision and linearity for pharmaceutical analysis.

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary electrophoresis (CE) is a powerful separation technique.
  • Ensuring accuracy and reproducibility in CE assays is crucial for reliable analytical results.

Purpose of the Study:

  • To identify factors influencing the accuracy of capillary electrophoresis assays.
  • To propose and demonstrate methods for improving the reproducibility and reliability of CE assays.

Main Methods:

  • General factors affecting CE assay accuracy were analyzed.
  • Specific CE assays were developed and validated for quinobene and suramin.
  • Reproducibility, accuracy, and linearity were evaluated.

Main Results:

  • Developed CE assays exhibited high reproducibility with relative standard deviation (RSD) below 2%.

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  • Assay accuracy was confirmed with errors less than 2%.
  • Excellent linearity was observed over a concentration range of 1-800 µg/mL (r² = 0.999).

    • Conclusions:

    • The suggested methods effectively improve the reproducibility and reliability of CE assays.
    • The developed CE methods for quinobene and suramin are accurate, reproducible, and linear.
    • This work provides a framework for enhancing the performance of CE analyses in various applications.