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Related Experiment Videos

Application of electrokinetic chromatography to pharmaceutical analysis

H Nishi1, S Terabe

  • 1Analytical Chemistry Research Laboratory, Tanabe Seiyaku Co. Ltd., Osaka, Japan.

Journal of Pharmaceutical and Biomedical Analysis
|November 1, 1993
PubMed
Summary

Micellar electrokinetic chromatography (MEKC) separates neutral and ionic compounds using surfactants. This technique is effective for pharmaceutical analysis, including drug enantiomer separation and direct plasma assays.

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Electrokinetic chromatography (EKC) is a branch of capillary electrophoresis for separating neutral solutes.
  • Micellar electrokinetic chromatography (MEKC) is a popular EKC mode using ionic surfactants above their critical micelle concentration (CMC).

Purpose of the Study:

  • To summarize the separation principles and characteristics of MEKC.
  • To highlight MEKC applications in pharmaceutical analysis.

Main Methods:

  • Utilizing micellar electrokinetic chromatography (MEKC) with ionic surfactants.
  • Modifying buffer composition and surfactants to alter separation selectivity.
  • Employing chiral surfactants or additives for enantiomeric separations.

Main Results:

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  • MEKC effectively separates electrically neutral, non-ionic, and ionic solutes.
  • Separation selectivity is easily manipulated by adjusting buffer and surfactant properties.
  • Direct enantiomeric separations and direct assays of drugs in plasma were successfully achieved.

Conclusions:

  • MEKC is a versatile technique for pharmaceutical analysis, applicable to various drug types.
  • MEKC facilitates direct enantiomeric separation and drug determination in biological matrices like plasma.