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[Expression of platelet derived growth factor receptors in cultivated lens epithelial cells]
M Knorr1, K Wunderlich, K P Steuhl
1Universitäts-Augenklinik Tübingen.
Abstract:
It has been shown that PDGF plays a role in the regulation of lens growth and differentiation. PDGF occurs in vivo as a homodimer or heterodimer of the two polypeptide chains A and B. These isoforms bind with different affinities to two distinct receptor types, termed alpha and beta. In order to identify the different PDGF receptors on cultured bovine lens epithelial cells (BLEC), we performed receptor binding studies, using 125I-labelled PDGF isoforms. Analysis of our data revealed that BLEC expressed approximately 35,000 PDGF-BB binding sites (KD = 21 ng/ml) and 9000 PDGF-AB binding sites (KD = 11 ng/ml), but only 4800 PDGF-AA binding sites (KD = 7 ng/ml). This study represents the first demonstration that lens epithelial cells express PDGF receptors.
Insights
Platelet-derived growth factor (PDGF) receptors are present on bovine lens epithelial cells (BLEC). This study is the first to demonstrate PDGF receptor expression in lens epithelial cells.
Area of Science:
- Cell biology
- Ophthalmology
- Molecular biology
Context:
- Platelet-derived growth factor (PDGF) is crucial for regulating lens growth and differentiation.
- PDGF exists as A and B polypeptide chains, forming homodimers or heterodimers.
- These isoforms exhibit varying affinities for two receptor types: alpha and beta.
Purpose:
- To identify and characterize PDGF receptor types expressed on cultured bovine lens epithelial cells (BLEC).
Summary:
- Receptor binding studies were conducted using 125I-labelled PDGF isoforms (PDGF-AA, PDGF-AB, PDGF-BB).
- BLEC demonstrated approximately 35,000 PDGF-BB binding sites (KD = 21 ng/ml), 9,000 PDGF-AB binding sites (KD = 11 ng/ml), and 4,800 PDGF-AA binding sites (KD = 7 ng/ml).
Impact:
- This research provides the first evidence of PDGF receptor expression in lens epithelial cells.
- Understanding PDGF signaling in the lens may offer insights into lens development and disease.