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Energy conservation in Bacillus megaterium

A J Downs, C W Jones

    Archives of Microbiology
    |October 27, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Bacillus megaterium strains D440 and M utilize distinct respiratory chain components for energy conservation. Their proton translocation mechanisms and growth yields were characterized, revealing differences under various nutrient limitations.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Cellular Respiration

    Background:

    • Investigating energy conservation in Bacillus megaterium is crucial for understanding microbial metabolism.
    • Previous studies on Bacillus megaterium respiration have not fully elucidated the specific proton translocation loops involved.

    Purpose of the Study:

    • To characterize the respiratory chain energy conservation systems in Bacillus megaterium strains D440 and M.
    • To determine the proton translocation stoichiometry (H+/O ratios) and molar growth yields (Y02, YATP) under different growth conditions.

    Main Methods:

    • Growth of Bacillus megaterium strains in batch and continuous cultures.
    • Analysis of respiratory membrane cytochromes.
    • Measurement of whole-cell H+/O ratios with specific substrates.

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  • In situ measurement of respiratory activities as a function of dilution rate.

    • Main Results:

    • Respiratory membranes contained distinct cytochrome compositions (b, aa3, o in D440; b, c, a, o in M).
    • H+/O ratios indicated proton translocating loops 1 and 2 in D440, and loops 2 and 3 in M.
    • Molar growth yields (Y02) were approximately 50 g cells/mole oxygen, with YATP values of 12.7 and 10.8 g cells/mole ATP for D440 and M, respectively.

    Conclusions:

    • Bacillus megaterium strains D440 and M possess different proton translocation pathways in their respiratory chains.
    • Energy for maintenance is low in energy-limited cultures but increases significantly with nitrogen limitation, potentially due to uncoupling.