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Sequence of a cDNA encoding the p53 protein in rhesus monkey (Macaca mulatta)

H D Kay1, C P Mountjoy, G Wu

  • 1Department of Internal Medicine, University of Nebraska Medical Center, Omaha 68198-6840.

Gene
|January 28, 1994
PubMed

Insights

The Rhesus monkey p53 gene sequence shows over 95% identity to humans, supporting its use in cancer research and antisense oligodeoxyribonucleotide drug development for acute myelogenous leukemia.

Area of Science:

  • Genomics
  • Molecular Biology
  • Primate Models

Background:

  • The p53 tumor suppressor gene is crucial in human cancer.
  • Antisense oligodeoxyribonucleotides are a promising therapeutic strategy for various cancers.
  • Rhesus monkeys are valuable preclinical models for human diseases.

Purpose of the Study:

  • To sequence the Rhesus monkey p53 gene.
  • To evaluate the Rhesus monkey as a model for human cancer research, particularly for antisense oligodeoxyribonucleotide therapies.
  • To compare Rhesus monkey p53 sequences with human and other primate sequences.

Main Methods:

  • DNA sequencing of the p53 gene in Rhesus monkeys (Macaca mulatta).
  • Sequence alignment and identity comparison with human and African green monkey p53 sequences.
  • Deduction and comparison of amino acid sequences.

Main Results:

  • A 2184-nucleotide sequence of the Rhesus monkey p53 gene was determined.
  • Greater than 95% sequence identity was found between Rhesus monkey and human p53.
  • The deduced amino acid sequence showed high conservation with human p53, with only 18 differences in 393 amino acids.

Conclusions:

  • The Rhesus monkey p53 gene is highly homologous to the human gene.
  • This high conservation supports the utility of Rhesus monkeys as a preclinical model for human cancers.
  • The Rhesus monkey model is suitable for testing novel antisense oligodeoxyribonucleotides for conditions like acute myelogenous leukemia.

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