Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleotide sequence corrections of the uidA open reading frame encoding beta-glucuronidase

H R Schlaman1, E Risseeuw, M E Franke-van Dijk

  • 1Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratory, The Netherlands.

Gene
|January 28, 1994
PubMed
Summary

Researchers identified two genetic differences in the uidA gene, which codes for beta-glucuronidase. One difference is a silent mutation, while the other alters an amino acid, potentially impacting enzyme function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The lysopinedehydrogenase gene used as a marker for the selection of octopine crown gall cells.

Plant molecular biology·2013
Same author

Studies on the structure of cointegrates between octopine and nopaline Ti-plasmids and their tumour-inducing properties.

Plant molecular biology·2013
Same author

Clonal analysis of heterogeneous crown gall tumor tissues induced by wild-type and shooter mutant strains ofAgrobacterium tumefaciens-expression of T-DNA genes.

Plant molecular biology·2013
Same author

Silent T-DNA genes in plant lines transformed by Agrobacterium tumefaciens are activated by grafting and by 5-azacytidine treatment.

Plant molecular biology·2013
Same author

Tumor formation on plants by mixtures of attenuated Agrobacterium tumefaciens T-DNA mutants.

Plant molecular biology·2013
Same author

Non-oncogenic plant vectors for use in the agrobacterium binary system.

Plant molecular biology·2013

Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • The uidA gene encodes the enzyme beta-glucuronidase, a widely used reporter in molecular biology.
  • Previous nucleotide sequences of uidA have been published.

Purpose of the Study:

  • To re-sequence a portion of the uidA gene.
  • To identify any discrepancies with previously reported sequences.

Main Methods:

  • DNA sequencing of a part of the uidA open reading frame.

Main Results:

  • Two differences were detected compared to the previously reported uidA nucleotide sequence.
  • One identified difference was a silent mutation.
  • The second difference resulted in an amino acid substitution (Glutamic acid to Glutamine at position 279).

Related Experiment Videos

Conclusions:

  • The re-sequencing revealed variations in the uidA gene sequence.
  • These variations, particularly the amino acid substitution, may warrant further investigation into their functional implications.