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Published on: September 5, 2010
Silicon analysis in biological specimens by direct current plasma-atomic emission spectroscopy
1National Medical Services, Inc., Willow Grove, PA 19090.
Journal of Analytical Toxicology
|January 1, 1994
Summary
A new method accurately measures total elemental silicon in biological fluids using direct current plasma-atomic emission spectrometry (DCP-AES). This technique establishes normal silicon ranges in blood, serum, plasma, and urine for clinical relevance.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Clinical Chemistry
Background:
- Elemental silicon is present in biological fluids.
- Accurate measurement of silicon is crucial for understanding its role in health and disease.
- Existing methods may lack sensitivity or specificity for biological samples.
Purpose of the Study:
- To present a novel method for quantifying total elemental silicon concentrations in biological fluids.
- To establish reference ranges for silicon in human blood, serum, plasma, and urine.
- To explore the method's potential in diagnosing silicone-related health conditions.
Main Methods:
- Direct current plasma-atomic emission spectrometry (DCP-AES) was employed for elemental silicon determination.
- The method was validated for linearity and detection limits in various biological matrices.
- Population-based studies were conducted to determine silicon concentration ranges.
Main Results:
- The DCP-AES method demonstrated linearity up to 30 µg/mL in blood, serum, plasma, and urine.
- A low detection limit of 0.2 µg/mL was achieved.
- Normal population ranges were established, with over 50% of blood, plasma, and serum samples showing concentrations below 0.5 µg/mL.
Conclusions:
- The developed DCP-AES method provides a reliable means for measuring total elemental silicon in biological fluids.
- Established population ranges offer valuable reference points for clinical interpretation.
- The method holds promise for investigating the clinical significance of silicon in various health and disease states.
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