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Related Experiment Videos

Fine filaments in lymphatic endothelial cells

J M Lauweryns, J Baert, W De Loecker

    The Journal of Cell Biology
    |January 1, 1976
    PubMed
    Summary

    Lymphatic endothelial cells possess actin-like filaments, suggesting a contractile system. These findings reveal a potential mechanism for cellular movement and tissue remodeling in lymphatic vessels.

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    Area of Science:

    • Cell Biology
    • Biochemistry
    • Microscopy

    Background:

    • Cytoplasmic filaments resembling muscle myofilaments are found in various cell types.
    • Some of these filaments react with heavy meromyosin (HMM), indicating the presence of actin.

    Purpose of the Study:

    • To investigate the fine structure of intracytoplasmic filaments in lymphatic endothelial cells.
    • To determine if these filaments react with heavy meromyosin (HMM) and EDTA, suggesting a contractile system.

    Main Methods:

    • Electron microscopy was used to examine the fine structure of lymphatic endothelial cells.
    • Incubation with heavy meromyosin (HMM) and EDTA was performed to assess filament reactions.

    Main Results:

    • Fine intracytoplasmic filaments were observed in lymphatic endothelial cells.
    • These filaments showed reactions consistent with actin when incubated with HMM and EDTA.

    Conclusions:

    • Lymphatic endothelial cells contain actin-like filaments.
    • These filaments likely form a contractile system within the cells, potentially influencing lymphatic function.

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