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Leucine synthesis in spinach chloroplasts: partial characterization of 2-isopropylmalate synthase
1Botanisches Institut, Tierärztlichen Hochschule Hannover, Germany.
Biological Chemistry Hoppe-Seyler
|December 1, 1993
Abstract:
Leucine synthesis was found to be localized in chloroplasts of higher plants. The introductory enzyme of the isopropylmalate pathway, the 2-isopropylmalate synthase, was strongly feedback-regulated by leucine. The pH optimum of the enzyme was between 7.0 and 9.0. The Km values were 5 microM for acetyl-CoA and 75 microM for 2-oxoisovalerate.