Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solution structure of pyoverdin GM-II

G Mohn1, P Koehl, H Budzikiewicz

  • 1Centre National de la Recherche Scientifique, Institut de Biologie Moleculaire et Cellulaire, Strasbourg, France.

Biochemistry
|March 15, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

INCIDENTAL INTRAOSSEOUS CALCANEAL LIPOMA IN A PATIENT SUFFERING FROM PLANTARFASZIITIS.

Georgian medical news·2022
Same author

INTRAMUSCULAR MYXOMA OF THE BUTTOCK- A CASE REPORT.

Georgian medical news·2022
Same author

The importance of structural softening for the evolution and architecture of passive margins.

Scientific reports·2016
Same author

Selected reviews in mass spectrometric topics.

Mass spectrometry reviews·2016
Same author

Selected reviews on mass spectrometric topics.

Mass spectrometry reviews·2016
Same author

Selected reviews on mass spectrometric topics.

Mass spectrometry reviews·2016

The three-dimensional structure of ferri-pyoverdin GM-II from Pseudomonas fluorescens was determined using Nuclear Magnetic Resonance (NMR) methods. This research provides crucial insights into the molecular conformation of this important siderophore.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Microbiology

Background:

  • Ferri-pyoverdin GM-II is a siderophore produced by Pseudomonas fluorescens.
  • Understanding the structure of siderophores is crucial for their biological function.

Purpose of the Study:

  • To determine the three-dimensional structure of ferri-pyoverdin GM-II in solution.
  • To elucidate the conformational properties of the siderophore.

Main Methods:

  • Nuclear Magnetic Resonance (NMR) spectroscopy was applied to the Ga3+ analogue.
  • Distance geometry calculations were performed using FILMAN with interproton distances and coupling constants.
  • Conformational analysis included energy minimization with MM2 and AMBER.

Main Results:

Related Experiment Videos

  • The three-dimensional solution structure of ferri-pyoverdin GM-II was successfully determined.
  • Back-calculation of NOESY spectra confirmed the agreement between the calculated structures and experimental data.

Conclusions:

  • The study provides a detailed structural model for ferri-pyoverdin GM-II.
  • The determined structure is consistent with the experimental NMR data, validating the methodology.