Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cholesterol's interfacial interactions with galactosylceramides

S Ali1, J M Smaby, H L Brockman

  • 1Hormel Institute, University of Minnesota, Austin 55912.

Biochemistry
|March 15, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-time measurement of solute partitioning to lipid monolayers.

Analytical biochemistry·2005
Same author

Peripheral protein adsorption to lipid-water interfaces: the free area theory.

Biophysical journal·2005
Same author

Regulation of lipases by lipid-lipid interactions: implications for lipid-mediated signaling in cells.

Chemistry and physics of lipids·2003
Same author

Tyrosine hydroxylase protein content in the medulla oblongata of the foetal sheep brain increases in response to acute but not chronic hypoxia.

Neuroscience letters·2001
Same author

Effects of chronic and acute methylphenidate hydrochloride (Ritalin) administration on locomotor activity, ultrasonic vocalizations, and neuromotor development in 3- to 11-day-old CD-1 mouse pups.

Developmental psychobiology·2001
Same author

Cost effectiveness of eptifibatide in acute coronary syndromes; an economic analysis of Western European patients enrolled in the PURSUIT trial. The Platelet IIa/IIb in unstable Angina: Receptor Suppression Using Integrilin Therapy.

European heart journal·2001

Acyl structure significantly impacts galactosylceramide (GalCer) interactions with cholesterol. Cholesterol strongly condenses liquid-disordered GalCer, influencing lipid phase behavior and membrane properties.

Area of Science:

  • Biochemistry
  • Lipid Biophysics
  • Membrane Biology

Background:

  • Galactosylceramide (GalCer) is a key sphingolipid in biological membranes.
  • The acyl chain structure of GalCer influences its physical properties and interactions.
  • Understanding GalCer-cholesterol interactions is crucial for deciphering membrane organization.

Purpose of the Study:

  • To investigate how the acyl chain structure of galactosylceramide (GalCer) affects its interaction with cholesterol.
  • To determine the impact of GalCer acyl chain saturation and length on cholesterol's condensing effect.
  • To compare cholesterol's condensation effect on GalCer versus sphingomyelin.

Main Methods:

  • Synthesis of chain-pure GalCer species with varying saturated and unsaturated acyl chains.

Related Experiment Videos

  • Measurement of force-area isotherms for mixed cholesterol/GalCer films at 24°C.
  • Determination of average molecular area and compressibility as a function of film composition.

    • Main Results:

    • Cholesterol exerts a marked condensing effect on liquid-disordered GalCer, irrespective of acyl chain saturation.
    • Maximum condensation of liquid-disordered GalCer by cholesterol occurs at 0.3-0.4 mole fraction.
    • Cholesterol's condensation effect is greater on sphingomyelin than on liquid-expanded GalCer species.

    Conclusions:

    • Acyl structure is a critical determinant of galactosylceramide's (GalCer) interaction with cholesterol.
    • Cholesterol significantly modulates the phase behavior of liquid-disordered GalCer.
    • Differences in cholesterol condensation suggest distinct interfacial interactions between GalCer and sphingomyelin species.