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A new 2D NMR method for measurement of JHH coupling constants
M D Sørensen1, J J Led, O W Sørensen
1Department of Chemistry, University of Copenhagen, Denmark.
Journal of Biomolecular NMR
|January 1, 1994
Abstract:
A new 2D NMR pulse sequence for E.COSY-type measurement of J(HH) coupling constants is introduced. It exploits a heteronuclear spin, e.g., 13C, for displacement in the omega(1) frequency dimension via a large heteronuclear J coupling. The experiment is demonstrated by application to a heptapeptide at the natural abundance 13C level. It is suitable, for example, for measurement of 3J(HH) and 4J(HH) coupling constants in peptides and proteins.