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Identification of the Methanococcus voltae S-layer structural gene

J Konisky1, D Lynn, M Hoppert

  • 1Department of Microbiology, University of Illinois, Urbana 61802.

Journal of Bacteriology
|March 1, 1994
PubMed
Summary

The gene previously thought to encode Methanococcus voltae P-type ATPase is actually the structural gene for the M. voltae S-layer protein. This finding clarifies the genetic basis of S-layer protein production in this archaeon.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Archaea Research

Background:

  • Methanococcus voltae is an archaeon known for its S-layer protein.
  • A previously identified gene was thought to encode a P-type ATPase.
  • The precise genetic origin of the S-layer protein was under investigation.

Purpose of the Study:

  • To identify the correct structural gene for the Methanococcus voltae S-layer protein.
  • To resolve the discrepancy regarding the function of a previously cloned gene.

Main Methods:

  • N-terminal sequencing of S-layer protein prepared via two independent methods.
  • Comparison of protein sequence data with nucleotide sequence of the cloned gene.
  • Immunocytochemical localization of the S-layer protein antigen.

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Main Results:

  • The N-terminal sequence of the S-layer protein matched the sequence derived from the cloned gene.
  • The gene previously identified as encoding P-type ATPase is confirmed as the S-layer protein gene.
  • Immunocytochemistry confirmed the localization of the S-layer protein.

Conclusions:

  • The gene in question is the structural gene for the Methanococcus voltae S-layer protein, not a P-type ATPase.
  • This study corrects previous functional assignments for a cloned gene in M. voltae.
  • The findings provide a clear genetic understanding of S-layer protein synthesis in M. voltae.