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Related Experiment Videos

Complete cDNA encoding human phospholipid transfer protein from human endothelial cells

J R Day1, J J Albers, C E Lofton-Day

  • 1Department of Medicine, University of Washington, Seattle 98103.

The Journal of Biological Chemistry
|March 25, 1994
PubMed
Summary

Researchers purified human phospholipid transfer protein (PLTP) and isolated its full-length cDNA. This protein shows homology to other lipid-binding proteins, suggesting a role in lipid metabolism.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Phospholipid transfer protein (PLTP) plays a role in lipoprotein metabolism.
  • Understanding PLTP's structure and function is crucial for lipid research.

Purpose of the Study:

  • To purify human PLTP.
  • To isolate and characterize the full-length cDNA encoding human PLTP.
  • To determine the gene's chromosomal location and protein's homology.

Main Methods:

  • Protein purification from human plasma.
  • Degenerate primer design for Polymerase Chain Reaction (PCR).
  • cDNA library screening and sequencing.
  • Northern blot analysis.
  • Gene mapping using somatic cell hybrid panels.

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Main Results:

  • Purification of an 81 kDa protein identified as PLTP.
  • Isolation of a 1,750 bp full-length cDNA encoding a 476-residue mature protein.
  • Identification of a wide tissue distribution for the 1.8 kb mRNA transcript.
  • Mapping of the PLTP gene to chromosome 20.
  • Homology of PLTP to cholesteryl ester transfer protein, lipopolysaccharide-binding protein, and bactericidal permeability increasing protein.

Conclusions:

  • The full-length cDNA for human PLTP has been isolated and characterized.
  • Human PLTP is encoded by a gene on chromosome 20.
  • PLTP shares structural similarities with other lipid-binding proteins, suggesting conserved functions.