Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isoprenylated proteins in myelin

L Sepp-Lorenzino1, P S Coleman, J N Larocca

  • 1SKI Program in Cell Biology, Memorial Sloan-Kettering Cancer Center, New York, New York.

Journal of Neurochemistry
|April 1, 1994
PubMed
Summary

Lovastatin treatment of rat brainstem slices reveals prenylated proteins in myelin. Inhibiting mevalonate (MVA) synthesis selectively impacts myelin protein production and assembly.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neonatal undernutrition may affect the delivery of sulfatides from perikarya of oligodendrocytes and golgi-related membranes.

Neurochemistry international·2010
Same author

Transport of sulfatides towards myelin. Effect of colchicine, monensin and calcium on their intracellular translocation.

Neurochemistry international·2010
Same author

Isolation of oligodendroglial cells from young and adult whole rat brains using an in situ generated percoll density gradient.

Neurochemistry international·2010
Same author

Interaction of Rab31 and OCRL-1 in oligodendrocytes: its role in transport of mannose 6-phosphate receptors.

Journal of neuroscience research·2009
Same author

Transport of mannose-6-phosphate receptors from the trans-Golgi network to endosomes requires Rab31.

Experimental cell research·2009
Same author

Challenges and opportunities for local and systemic delivery of siRNA and antisense oligonucleotides.

Clinical pharmacology and therapeutics·2008

Area of Science:

  • Neuroscience
  • Biochemistry
  • Cell Biology

Background:

  • Myelin is a crucial lipid-rich sheath insulating nerve fibers.
  • Prenylation, a post-translational modification, is essential for the function of many proteins.
  • The role of prenylation in myelin protein synthesis and assembly is not fully understood.

Purpose of the Study:

  • To investigate the incorporation of mevalonate into myelin-associated proteins.
  • To identify prenylated proteins within rat brainstem myelin.
  • To determine the effect of lovastatin on myelin protein synthesis and prenylation.

Main Methods:

  • Incubation of rat brainstem slices with [3H]-mevalonate and lovastatin.
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for protein separation.
  • Immunoprecipitation to identify specific prenylated proteins.

Main Results:

  • [3H]-mevalonate was incorporated into 47, 21-27, and 8 kDa myelin-associated proteins.
  • The 47-kDa protein identified as 2'-3'-cyclic nucleotide phosphodiesterase; 8-kDa protein as gamma subunit of G protein.
  • Lovastatin reduced myelin prenylated proteins, increased cytosolic levels, and altered nonprenylated myelin protein synthesis.

Conclusions:

  • Prenylation is a significant post-translational modification for specific myelin proteins.
  • Mevalonate availability is critical for the proper synthesis and assembly of myelin proteins.
  • Lovastatin's impact suggests a selective vulnerability in myelinogenesis to disruptions in the mevalonate pathway.

Related Experiment Videos