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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
Published on: March 12, 2017
Polishing methods for the lens capsule: histology and scanning electron microscopy
C F Mathey1, T B Kohnen, H J Ensikat
1Klinik Dardenne, Bonn, Germany.
Abstract:
Capsule opacification occurs when lens epithelial cells remains on the capsule after extracapsular cataract surgery. The cells divide and form fibers. The best way to prevent opacification is to remove all epithelial cells from the capsule. Our study compared the efficacy of different mechanical polishing methods in eyes in which cataracts were removed by endocapsular phacoemulsification through a miniature capsulorhexis. The capsular bag was polished using one of five techniques: metallic scrapers, silicone scrapers, Rentsch capsule curettes, irrigation/aspiration tip, or ultrasound irrigation/aspiration tip. Then a circular central portion of the anterior capsule was removed and processed for histology and scanning electron microscopy. A control group comprised unpolished central anterior capsules obtained by capsulorhexis before or after endophacoemulsification. The cleanest capsules were obtained by polishing with the ultrasound irrigation/aspiration tip.
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