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Related Experiment Videos

Calcium buffering in bursting Helix pacemaker neurons

T H Müller1, L D Partridge, D Swandulla

  • 1Max-Planck-Institute für biophysikalische Chemie, Abteilung Membranbiophysik, Göttingen, Germany.

Pflugers Archiv : European Journal of Physiology
|December 1, 1993
PubMed
Summary

Researchers measured intracellular calcium changes in snail neurons using fura-2. They determined the endogenous calcium buffer capacity, finding it robust even at high calcium levels.

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Area of Science:

  • Neuroscience
  • Cellular Physiology

Background:

  • Pacemaker neurons generate rhythmic electrical activity essential for physiological functions.
  • Calcium ions (Ca) play a critical role in neuronal excitability and signaling.
  • Understanding intracellular Ca dynamics and buffering is key to comprehending neuronal function.

Purpose of the Study:

  • To quantify the endogenous Ca buffer capacity in bursting pacemaker neurons of Helix pomatia.
  • To investigate the relationship between Ca influx and intracellular Ca buffering.
  • To determine if neuronal Ca buffers are saturated at physiologically relevant Ca concentrations.

Main Methods:

  • Voltage-clamping of bursting pacemaker neurons from Helix pomatia.
  • Simultaneous recording of Ca currents and intracellular Ca concentrations using fura-2 and digital imaging.

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  • Manipulating fura-2 concentration to assess endogenous Ca buffering capacity.

    • Main Results:

    • Depolarizing steps induced spatially non-uniform increases in intracellular Ca.
    • Measured Ca signals decayed with a time constant of approximately 5 seconds.
    • Endogenous Ca buffer capacity was determined to be approximately 480 buffered ions per free Ca ion.
    • Buffer capacity remained consistent across tested Ca influx levels, indicating no saturation up to 1 microM.

    Conclusions:

    • Helix pomatia pacemaker neurons possess a significant endogenous Ca buffering system.
    • This buffering system is effective in modulating intracellular Ca transients during neuronal activity.
    • The endogenous Ca buffer is not saturated at intracellular Ca concentrations up to 1 microM, suggesting a substantial capacity to buffer Ca influx.