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Folate metabolism in normal peripheral leukocytes in children
Acta Histochemica
|January 1, 1975
Summary
Dihydrofolate reductase activity in children's leukocytes is present and varies significantly. This enzyme activity differs by cell type, age, sex, and individual children, especially during puberty.
Area of Science:
- Biochemistry
- Hematology
- Pediatric enzymology
Background:
- Dihydrofolate reductase (DHFR) is crucial for cell proliferation.
- Understanding DHFR activity in pediatric leukocytes is important for hematological and immunological studies.
Purpose of the Study:
- To investigate the histochemical activity of dihydrofolate reductase in leukocytes of children.
- To identify variations in DHFR activity across different leukocyte types and developmental stages.
Main Methods:
- Histochemical staining method was employed to assess DHFR activity.
- Analysis was performed on blood leukocytes from children, including neutrophils, eosinophils, lymphocytes, and monocytes.
Main Results:
- Blood leukocytes demonstrated positive dihydrofolate reductase enzymic activity.
- Significant enzymic variability was observed in neutrophils, eosinophils, and lymphocytes, particularly during puberty and early adolescence.
- Monocytes showed less variability compared to other cell types.
Conclusions:
- Dihydrofolate reductase is enzymatically active in pediatric leukocytes.
- DHFR activity exhibits considerable variability influenced by leukocyte type, age, sex, and individual differences, especially during adolescent development.