LR Gold and LR White embedding of lung tissue for immunoelectron microscopy

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Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography
07:41

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Published on: October 17, 2018

M Kasper1, A Migheli

  • 1Institute of Pathology, Medical Academy Carl Gustav Carus, Dresden, Germany.

Acta Histochemica
|December 1, 1993
PubMed

The immunogold technique in conjunction with LR Gold or LR White embedding was used to localize different antigens in normal rat and mini pig lung tissues. Lycopersicon esculentum and Maclura pomifera lectins and diverse cytoskeletal antigens such as cytokeratins, beta-tubulin, smooth muscle-actin, tropomyosin and villin were labelled well. Factor VIII-related antigen was found in Weibel-Palade bodies of endothelial cells. A 10 kDa Clara cell protein was localized in Clara cells. The morphological preservation of LR Gold-embedded tissue was superior to that of LR White-embedded tissues, but significant differences in antigenicity were not seen.

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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.