Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A novel immunosensor for herpes viruses

B König1, M Grätzel

  • 1Institut de Chimie Physique II, Ecole Polytechnique Fédérale de Lausanne, Switzerland.

Analytical Chemistry
|February 1, 1994
PubMed
Summary

A novel reusable piezoelectric immunosensor detects five human herpes viruses. This biosensor demonstrates high stability and reusability for reliable viral diagnostics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Family History and Solar Insolation in Bipolar I Disorder.

Acta psychiatrica Scandinavica·2026
Same author

Tuning biological processes <i>via</i> co-solutes: from single proteins to protein condensates - the case of α-elastin condensation.

Chemical science·2025
Same author

Sociability increases survival of adult female giraffes.

Proceedings. Biological sciences·2021
Same author

TREM-1 and TREM-2 Expression on CD14<sup>+</sup> Cells in Bronchoalveolar Lavage Fluid in Pulmonary Sarcoidosis and Hypersensitivity Pneumonitis in the Context of T Cell Immune Response.

Mediators of inflammation·2020
Same author

X-ray fluorescence analysis of metal distributions in cryogenic biological samples using large-acceptance-angle SDD detection and continuous scanning at the Hard X-ray Micro/Nano-Probe beamline P06 at PETRA III.

Journal of synchrotron radiation·2019
Same author

Characterization of the in situ pellicle ultrastructure formed under the influence of bovine milk and milk protein isolates.

Archives of oral biology·2019

Area of Science:

  • Biomedical Engineering
  • Immunosensor Technology
  • Virology

Background:

  • Human herpes viruses pose significant health challenges.
  • Accurate and rapid detection methods are crucial for diagnosis and management.
  • Existing diagnostic tools may have limitations in terms of speed, cost, or reusability.

Purpose of the Study:

  • To develop a reusable piezoelectric immunosensor for detecting five specific human herpes viruses.
  • To utilize synthetic peptides and monoclonal antibodies for virus-specific detection.
  • To evaluate the sensor's performance, including sensitivity, linearity, and long-term stability.

Main Methods:

  • Development of a piezoelectric immunosensor using a 10-MHz AT-cut crystal.
  • Immobilization of virus-specific monoclonal antibodies onto the crystal surface via protein A.
  • Detection of herpes simplex viruses types 1 and 2, varicella-zoster virus, Epstein-Barr virus, and cytomegalovirus.
  • Analysis of frequency changes in response to viral load.

Main Results:

  • The immunosensor detected a viral load ranging from 5 x 10(4) to 1 x 10(9) viruses.
  • A linear frequency change was observed correlating with viral concentration.
  • The modified crystal exhibited long-term stability of 8 weeks when stored dry.
  • The sensor maintained activity for at least 18 reuses without significant loss.

Conclusions:

  • A reusable piezoelectric immunosensor was successfully developed for detecting five human herpes viruses.
  • The sensor offers a stable, sensitive, and reusable platform for viral detection.
  • This technology holds promise for improved diagnostic capabilities in virology.

Related Experiment Videos