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Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms
E D Kopczynski1, M M Bateson, D M Ward
1Department of Microbiology, Montana State University, Bozeman 59717.
Applied and Environmental Microbiology
|February 1, 1994
Abstract:
When PCR was used to recover small-subunit (SSU) rRNA genes from a hot spring cyanobacterial mat community, chimeric SSU rRNA sequences which exhibited little or no secondary structural abnormality were recovered. They were revealed as chimeras of SSU rRNA genes of uncultivated species through separate phylogenetic analysis of short sequence domains.