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Updated: Sep 11, 2026

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
Published on: July 14, 2017
Modulation of 12-0-tetradecanoylphorbol-13-acetate-induced epidermal ornithine decarboxylase activity by calcium and
C Dwivedi1, R K Baer, D M Jarvis
1College of Pharmacy, South Dakota State University, Brookings 57007-0099.
Abstract:
Calcium has been suggested to be an intracellular second messenger for ornithine decarboxylase (ODC) induction caused by 12-O-tetradecanoylphorbol-13-acetate (TPA). In the present study, the effects of dietary calcium supplement and calcium and verapamil injections on TPA-induced ODC activity in skin was investigated in CD-1 and SENCAR mouse. Dietary calcium supplement did not influence ODC induction caused by TPA. However, calcium injections enhanced the TPA-induced ODC activity in CD-1 and SENCAR mouse skin. Verapamil injections resulted in a significant decrease in TPA-induced ODC activity in CD-1 and SENCAR mice. These results suggest that calcium and calcium antagonists may play a role in skin tumor promotion caused by TPA.
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