Related Experiment Videos
Murine hybridomas secreting monoclonal antibodies reacting with Mlsa antigens
L Di Giorgi1, C De Matos, B A Ellis
1Immunology Department, Rayne Institute, St Thomas' Hospital, London, UK.
Murine monoclonal antibodies have been developed from spleens of AKR mice hyperimmunised with the monoclonal antibody against TCR-V beta 6. Two new monoclonal antibodies called AL-12-3 and AL-32-7 were able to specifically block Mlsa-induced but not MHC-induced mixed lymphocyte responses in a dose-dependent manner. Both antibodies are mouse IgG3 lambda chain isotype and bind to Mlsa-bearing lymphocytes from different strains of mice. The availability of such antibodies may well facilitate future research aimed at immunochemical and molecular characterization of Mls determinants.
Murine monoclonal antibodies have been developed from spleens of AKR mice hyperimmunised with the monoclonal antibody against TCR-V beta 6. Two new monoclonal antibodies called AL-12-3 and AL-32-7 were able to specifically block Mlsa-induced but not MHC-induced mixed lymphocyte responses in a dose-dependent manner. Both antibodies are mouse IgG3 lambda chain isotype and bind to Mlsa-bearing lymphocytes from different strains of mice. The availability of such antibodies may well facilitate future research aimed at immunochemical and molecular characterization of Mls determinants.