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Thyroxine-binding globulin biosynthesis in isolated monkey hepatocytes

D Glinoer, M C Gershengorn, J Robbins

    Biochimica Et Biophysica Acta
    |January 19, 1976
    PubMed
    Summary
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    Hepatocytes from Rhesus monkeys synthesize thyroxine-binding globulin (TBG). This study demonstrates TBG production and likely secretion by monkey liver cells over a 6-hour incubation period.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Endocrinology

    Background:

    • Thyroxine-binding globulin (TBG) is crucial for thyroid hormone transport.
    • Understanding TBG biosynthesis in non-human primates can provide insights into human physiology.

    Purpose of the Study:

    • To demonstrate and characterize thyroxine-binding globulin (TBG) biosynthesis in isolated Rhesus monkey hepatocytes.
    • To investigate the synthesis and potential secretion of TBG by these cells.

    Main Methods:

    • Isolation of hepatocytes from Rhesus monkeys via liver perfusion.
    • Measurement of radioactive leucine incorporation to assess protein synthesis.
    • Analysis of TBG in cell fractions and medium using immunoprecipitation and chromatography.

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    Main Results:

    • Hepatocytes maintained high survival rates and continuous protein synthesis during incubation.
    • Newly synthesized TBG was detected within 4 hours and increased over 6 hours.
    • A significant increase in TBG in the incubation medium suggests probable secretion.

    Conclusions:

    • Rhesus monkey hepatocytes are capable of synthesizing thyroxine-binding globulin (TBG).
    • Evidence suggests that TBG is secreted by these hepatocytes.
    • This model provides a valuable tool for studying TBG metabolism and regulation.