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Localization of disulfide bridges and free sulfhydryl groups in human eosinophil granule major basic protein
C Oxvig1, G J Gleich, L Sottrup-Jensen
1Department of Molecular Biology, University of Aarhus, Denmark.
FEBS Letters
|March 21, 1994
Abstract:
Eosinophil granule major basic protein (117 residues) is known to contain free sulfhydryl groups. Here we have located in the amino acid sequence the half-cystine residues present as cysteine, and identified those engaged in disulfide bridges. Of the 9 half-cystine residues, 5 are unpaired cysteines (Cys2, Cys23, Cys42, Cys64, and Cys96), while 4 form disulfides (Cys20-Cys115, and Cys92-Cys107).