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Meiotic mutants that cause a polar decrease in recombination on the X chromosome in Caenorhabditis elegans

S A Broverman1, P M Meneely

  • 1Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

Genetics
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

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Recessive mutations in Caenorhabditis elegans him genes disrupt X chromosome pairing and recombination, leading to increased nondisjunction. This affects gene mapping and suggests these genes initiate a crucial process on the X chromosome.

Area of Science:

  • Genetics
  • Developmental Biology
  • Molecular Biology

Background:

  • Recessive mutations in specific autosomal genes (him-1, him-5, him-8) in C. elegans hermaphrodites are known to cause high X chromosome nondisjunction.
  • This nondisjunction primarily affects the X chromosome, with no significant impact on autosomal chromosome segregation.

Purpose of the Study:

  • To investigate the relationship between X chromosome recombination and nondisjunction in him mutants.
  • To understand how mutations in him genes alter the distribution of recombination events along the X chromosome.

Main Methods:

  • Analysis of X chromosome nondisjunction rates in him-1, him-5, and him-8 mutant Caenorhabditis elegans.
  • Quantification and mapping of X chromosome recombination events in these mutants.

Related Experiment Videos

  • Comparison of recombination patterns between X chromosomes and autosomes.

    • Main Results:

    • Mutations in him-1, him-5, and him-8 result in reduced X chromosome recombination, which correlates with increased nondisjunction.
    • Recombination levels remain normal or elevated at the X chromosome's left end (pairing region) but decrease towards the right end.
    • The genetic map of the X chromosome in him mutants shows gene clustering due to reduced recombination, similar to autosomal maps.

    Conclusions:

    • The him genes are essential for a processive event initiating X chromosome pairing near the left end.
    • Altered recombination number and distribution in him mutants disrupt normal X chromosome segregation.
    • These findings provide insights into the mechanisms of chromosome pairing and recombination regulation.