Effect of brinolase on corneal endothelium

We investigated Brinolase, a substance producing enzymatic zonulysis, for effects on the physiological and anatomical function of the corneal endothelium in the specular microscope. Concentrations of 100 C2 units/ml perfused for ten minutes did not appear to damage the corneal endothelium. Longer perfusions or higher concentrations possibly produced alterations in the physiological functions of the endothelium, but no significant anatomic change was demonstrated. Longer perfusion times with high concentrations (1,600 C2 units/ml) resulted in the formation of holes which perforated the corneal stroma.