Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease of nerve Na+,K(+)-ATPase activity in the pathogenesis of human diabetic neuropathy

E Scarpini1, R Bianchi, M Moggio

  • 1Department of Neurology, Dino Ferrari Center, University of Milan, Italy.

Journal of the Neurological Sciences
|December 15, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasma microglial-derived extracellular vesicles are increased in frail patients with Mild Cognitive Impairment and exert a neurotoxic effect.

GeroScience·2023
Same author

Late-onset presentation and phenotypic heterogeneity of the rare R377W PSEN1 mutation.

European journal of neurology·2020
Same author

Kappa free light chains is a valid tool in the diagnostics of MS: A large multicenter study.

Multiple sclerosis (Houndmills, Basingstoke, England)·2019
Same author

Inflammatory expression profile in peripheral blood mononuclear cells from patients with Nasu-Hakola Disease.

Cytokine·2019
Same author

Human Schwann Cell Proliferation and IL-6 Production following TNF-α Stimulation in Vitro.

Annals of the New York Academy of Sciences·2017
Same author

Schwann Cell Apoptosis during Cell-Mediated Demyelination.

Annals of the New York Academy of Sciences·2017
Same journal

Distinguishing drug-target effects from natural lipid variation and incorporating imaging outcomes in Mendelian randomisation studies of multiple sclerosis.

Journal of the neurological sciences·2026
Same journal

Long-term social cognitive impairment after ischemic stroke: Frequency and association with lesion volume and location in an exploratory pilot study.

Journal of the neurological sciences·2026
Same journal

Subjective cognitive decline among U.S. Cancer survivors, 2017-2024: Prevalence, cancer-specific patterns, and associated factors.

Journal of the neurological sciences·2026
Same journal

Comment on "Comparative impact of mental and cardiovascular comorbidities on adverse outcomes in people with MS".

Journal of the neurological sciences·2026
Same journal

Comment on "Efficacy and safety of conventional immunosuppressant therapies in elderly patients with Neuromyelitis optica spectrum disorder: A target trial emulation study".

Journal of the neurological sciences·2026
Same journal

Increasing incidence of varicella-zoster virus meningitis in Japan, 2011-2022.

Journal of the neurological sciences·2026
See all related articles

Reduced sodium-potassium adenosine triphosphatase (Na+,K(+)-ATPase) activity is a key factor in diabetic neuropathy. This enzyme decrease in peripheral nerves contributes to nerve damage and may drive the condition

Area of Science:

  • Neurology
  • Biochemistry
  • Diabetology

Background:

  • Diabetic neuropathy is characterized by nerve damage in diabetes mellitus.
  • Decreased Na+,K(+)-ATPase activity is implicated in animal models of diabetic neuropathy.
  • The role of Na+,K(+)-ATPase in human diabetic neuropathy requires further investigation.

Purpose of the Study:

  • To investigate the activity of Na+,K(+)-ATPase in peripheral nerves of human patients with diabetic neuropathy.
  • To compare Na+,K(+)-ATPase activity and nerve fiber density in diabetic neuropathy versus non-diabetic neuropathy and normal controls.
  • To determine if reduced Na+,K(+)-ATPase activity is a cause or consequence of fiber loss in diabetic neuropathy.

Main Methods:

  • Peripheral nerve samples from 17 diabetic patients, 16 normal individuals, and 12 non-diabetic neuropathic patients were analyzed.

Related Experiment Videos

  • Ouabain-sensitive ATPase activity was measured using a spectrophotometric coupled-enzyme assay.
  • Histology, fiber teasing, electron microscopy, and morphometric analysis were performed to assess nerve structure and fiber density.

    • Main Results:

    • Na+,K(+)-ATPase activity was significantly lower (59%) in diabetic nerves compared to normal controls.
    • Nerves from non-diabetic neuropathic patients also showed reduced Na+,K(+)-ATPase activity (38%) and fiber density (22%).
    • In diabetic patients, decreased Na+,K(+)-ATPase activity did not correlate with reduced nerve fiber density, suggesting it's not solely secondary to fiber loss.

    Conclusions:

    • Reduced Na+,K(+)-ATPase activity is a significant finding in human diabetic neuropathy.
    • This enzymatic decrease appears to be a primary factor in the pathogenesis of diabetic neuropathy, not just a consequence of nerve damage.
    • The findings support the hypothesis that diminished Na+,K(+)-ATPase activity contributes to the development and persistence of diabetic neuropathy.