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[A biochemical study of creatine phosphokinase in the myocardium and its morphological changes in patients with acute
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Myocardial creatine phosphokinase (CPK) activity significantly decreases in patients with vascular abnormalities like aortic rupture. This reduction correlates with cardiomyocytic damage and hypoxia, indicating impaired heart muscle metabolism.
Area of Science:
- Cardiovascular Pathology
- Biochemistry
- Surgical Medicine
Background:
- Myocardial structure and metabolism are crucial for cardiac function.
- Creatine phosphokinase (CPK) is a key enzyme in myocardial energy metabolism.
- Vascular abnormalities can impact cardiac health.
Purpose of the Study:
- To investigate the relationship between myocardial structure, metabolism, and vascular abnormalities.
- To assess the activity of creatine phosphokinase (CPK) and redox enzymes in affected myocardial tissue.
Main Methods:
- Autopsy studies of patients from a surgical clinic.
- Biochemical analysis of CPK activity.
- Morphological examination of myocardial tissue, including cardiomyocytic lesions.
- Assessment of redox enzyme activity in muscle fiber fragmentation areas.
Main Results:
- A significant decrease in CPK activity was observed, particularly in patients with atherosclerotic aneurysm and abdominal aortic rupture.
- Morphological studies revealed cardiomyocytic and papillary muscle lesions.
- Reduced activity of redox enzymes was noted in areas of muscle fiber fragmentation.
- Vascular abnormalities were associated with progressive myocardial hypoxia and drastically decreased CPK and hydroxyreductase activity.
Conclusions:
- Surgical vascular abnormalities lead to progressive myocardial hypoxia.
- Decreased CPK and hydroxyreductase activity are indicators of myocardial damage in these conditions.
- Myocardial lesions and impaired energy metabolism are consequences of severe vascular disease.
Abstract:
The structure and metabolism of various myocardial portions, including the activity of creatine phosphokinase (CPK), were studied by using the autopsies of patients who had died in a surgical clinic. The biochemical studies demonstrated a decrease in CPK activity, which was pronounced in patients with atherosclerotic aneurysm and abdominal aortic rupture. The morphological studies revealed individual cardiomyocytic lesions, segmental lesions of papillary muscle. There was reduced activity of redox enzymes in the fragmentation areas of muscle fibers. Various surgical abnormality of vessels was accompanied by progressive myocardial hypoxia and drastically decreased activity of CPK and the tested hydroxyreductases.