[Comparative studies in the diagnosis of Chlamydia trachomatis infections]

Comparative evaluation of different methods for diagnosis of chlamydial infections was carried out. During the culture of the bacteria cycloheximide treated McCoy cell line was used, and elementary and reticulate bodies were detected by monoclonal antibodies with immunofluorescent (IF) method. Chlamydia specific IgM and IgG antibodies were determined with ELISA using S. minnesota mR 595 lipopolysaccharide (LPS) as antigen and by indirect IF with C. trachomatis L2 antigen. According to the results the LPS-ELISA developed in their laboratory proved to be an applicable method for the serodiagnosis of chlamydial infections parallel with the culture method.