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Halothane and isoflurane decrease calcium sensitivity and maximal force in human skinned cardiac fibers

B M Tavernier1, P J Adnet, M Imbenotte

  • 1Département d'Anesthésie-Réanimation Chirurgicale I, Hôpital B, Centre Hospitalier Régional Universitaire, Lille, France.

Anesthesiology
|March 1, 1994
PubMed
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Volatile anesthetics like halothane and isoflurane reduce calcium sensitivity and maximal force in human cardiac fibers. These findings in skinned cardiac fibers suggest a potential mechanism for their negative inotropic effects.

Area of Science:

  • Cardiology
  • Anesthesiology
  • Molecular Physiology

Background:

  • Conflicting reports exist on volatile anesthetics' direct effects on cardiac myofibrils in humans.
  • Previous studies primarily focused on mammalian species, leaving human cardiac protein responses unclear.

Purpose of the Study:

  • To investigate the direct impact of halothane and isoflurane on human cardiac contractile proteins.
  • To determine the effects of equianesthetic doses on Ca2+ sensitivity and maximal force in human skinned cardiac fibers.

Main Methods:

  • Human left ventricular muscle strips were obtained from cardiac surgery patients.
  • Sarcolemma and sarcoplasmic reticulum were disrupted to create skinned fibers.
  • Isometric tension was measured across varying Ca2+ concentrations (pCa) to assess Ca2+ sensitivity and maximal force.

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Main Results:

  • Both halothane and isoflurane dose-dependently decreased Ca2+ sensitivity and maximal force in human skinned cardiac fibers.
  • Anesthetics shifted pCa-tension curves to higher Ca2+ concentrations, reducing half-maximal activation.
  • No significant differences were observed between halothane and isoflurane at equianesthetic concentrations.

Conclusions:

  • Halothane and isoflurane directly impair Ca2+ sensitivity and maximal force in human cardiac fibers at 20°C.
  • These direct effects on contractile proteins may contribute to the negative inotropic effects of volatile anesthetics.
  • Further research is needed to confirm these effects at physiological temperatures.