Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Surface immune precipitation: a new method for rapid quantitative antigen analysis

D A Morris, M D Smith, J Greyson

    Journal of Immunological Methods
    |January 1, 1976
    PubMed
    Summary

    A novel Surface Immune Precipitation (SIP) method enables rapid, quantitative antigen analysis without lengthy incubation. This technique offers a faster alternative for detecting specific antibodies and measuring antigen concentration in samples.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The influence of light, gibberellic acid and CCC on sprout growth and mobilization of tuber reserves in the potato (Solanum tuberosum L.).

    Planta·2014
    Same author

    Distribution of (14)C-labelled sucrose in seedlings of Pisum sativum L. Treated with indoleacetic acid and kinetin.

    Planta·2014
    Same author

    The transport and metabolism of (14)C-labelled indoleacetic acid in intact pea seedlings.

    Planta·2014
    Same author

    Light and the transport and metabolism of indoleacetic acid in normal and albino dwarf pea seedlings.

    Planta·2014
    Same author

    Pathways of auxin transport in the intact pea seedling (Pisum sativum L.).

    Planta·2014
    Same author

    Auxin transport in intact pea seedlings (Pisum sativum L.): The inhibition of transport by 2,3,5-triiodobenzoic acid.

    Planta·2014

    Area of Science:

    • Immunology
    • Biochemistry
    • Analytical Chemistry

    Background:

    • Traditional antigen analysis methods often require lengthy incubation periods, delaying results.
    • Accurate and efficient quantitative antigen detection is crucial in various diagnostic and research settings.

    Purpose of the Study:

    • To introduce a novel, rapid technique for quantitative antigen analysis.
    • To present Surface Immune Precipitation (SIP) as a time-efficient alternative to existing methods.

    Main Methods:

    • Surface Immune Precipitation (SIP) involves applying an antigen droplet directly onto an antibody-containing gel.
    • The gel is supported by a reflecting substrate to enhance precipitate visibility.
    • Precipitate scattering intensity is measured using photodetection, correlating with antigen concentration.

    Related Experiment Videos

    Main Results:

    • SIP produces a visible opaque precipitate within minutes when specific antibodies are present.
    • A reflecting substrate significantly improves contrast between the precipitate and gel.
    • The method demonstrated good correlation with radial immunodiffusion for serum IgG analysis.

    Conclusions:

    • Surface Immune Precipitation (SIP) offers a rapid and effective method for quantitative antigen analysis.
    • The technique eliminates the need for long incubation times, improving efficiency.
    • SIP shows promise as a valuable tool in immunological assays and diagnostics.