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[Monoclonal antibodies in blood group serology. Part 2: MNS, Rh and other blood group systems]
H H Sonneborn1, W Dahr, W Helmbold
1Biotest AG, Forschungsabteilung, Dreieich.
Objective:
The introduction of monoclonal antibodies in routine blood group typing has also given rise to new findings. This paper was designed to demonstrate and clarify modifications relevant in practice.
Sources:
Our own experience in the production and application of monoclonal antibodies as well as the relevant literature are taken into consideration.
Results:
The development and production of murine and human monoclonal antibodies is described especially regarding the selection and the safety of clones. An update of availability and use of monoclonal antibodies in respect to the different blood group systems is given, with special reference to the reactivity of these new reagents with weak D-positive (Du) red blood cells.
Conclusion:
The application of monoclonal antibodies in blood group serology has improved the detection of weak variants and the availability of these reagents in general. Further improvements in this technology will make monoclonal antibodies also available for other blood group systems.