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Predicting submaximal oxygen consumption during treadmill running in mice

P Fernando1, A Bonen, L Hoffman-Goetz

  • 1Department of Kinesiology, Faculty of Applied Health Sciences, University of Waterloo, Canada.

Canadian Journal of Physiology and Pharmacology
|October 1, 1993
PubMed
Summary

Researchers developed a method to accurately predict oxygen consumption (VO2) in mice during treadmill exercise. This regression equation uses running speed and body weight, simplifying exercise physiology studies in rodents.

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Area of Science:

  • Exercise Physiology
  • Animal Models

Background:

  • Treadmill exercise is common in rodent studies.
  • Measuring oxygen consumption (VO2) in small, exercising animals is challenging.

Purpose of the Study:

  • Develop a regression equation to predict submaximal VO2 in mice during treadmill running.
  • Improve the ease of VO2 measurement in rodent exercise studies.

Main Methods:

  • Twenty female mice of three strains ran on a metabolic treadmill at speeds from 10-20 m/min.
  • Expired gas samples were collected and analyzed for O2 and CO2.
  • A regression equation was developed using running speed and body weight.

Main Results:

  • The developed equation accurately predicted submaximal VO2 (R2 = 0.85).

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  • Validation in an independent group showed a good correlation (r = 0.79) and low prediction error (+2.4 +/- 2.9%).
  • Measures of oxygen consumption were highly reliable (r = 0.96).

    • Conclusions:

    • A practical regression equation can predict submaximal oxygen consumption in mice during treadmill exercise.
    • This method simplifies VO2 assessment in rodent research.
    • The findings enhance the study of exercise physiology in mice.