Related Experiment Videos
Restriction endonuclease analysis of a porcine isolate of bovine herpesvirus type 1
E Várady1, T Tuboly, J B Derbyshire
1Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph.
Abstract:
Deoxyribonucleic acid (DNA) was extracted from bovine herpesvirus type 1 (BHV-1) isolated from a stillborn porcine fetus, from the Cooper reference strain of BHV-1, and from an Ontario bovine respiratory isolate. Each DNA was digested with the restriction endonucleases HindIII, EcoRI, HpaI and BamHI. Except for very minor differences in the patterns produced after digestion with EcoRI and HindIII, the DNA of the porcine isolate reacted in a similar manner to the bovine viruses, and it was concluded that the porcine virus is genetically similar to bovine isolates of BHV-1.